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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
19/09/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, C. F.; GUTIERREZ, D. L.; KREUZE, J. F.; ISKRA-CARUANA, M. L.; CHABANNES, M.; BARBOSA, A. C. O.; SANTOS, T. A.; SILVA, A. G. S.; SANTOS, R. M. F.; AMORIM, E. P.; OLIVEIRA, S. A. S. de; JESUS, O. N. de. |
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Afiliação: |
CLAUDIA FORTES FERREIRA, CNPMF; D. L. GUTIERREZ, INIA; J. F. KREUZE, CIP; M. L. ISKRA-CARUANA, CIRAD; M. CHABANNES, CIRAD; A. C. O. BARBOSA, UFRB; T. A. SANTOS, UFRB; A. G. S. SILVA, UFRB; R.M.F. SANTOS, UEFS; EDSON PERITO AMORIM, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ONILDO NUNES DE JESUS, CNPMF. |
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Título: |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n.3, 2019. |
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Idioma: |
Inglês |
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Notas: |
gmr18394 |
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Conteúdo: |
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. |
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Thesagro: |
Melhoramento Vegetal. |
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Thesaurus Nal: |
Breeding and Genetic Improvement. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202165/1/-rapid-plant-dna-and-rna-extraction-protocol-using-bench-drill.pdf
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Marc: |
LEADER 02135naa a2200289 a 4500
001 2112332
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
100 1 $aFERREIRA, C. F.
245 $aRapid plant DNA and RNA extraction protocol using a bench drill Brief Note.$h[electronic resource]
260 $c2019
500 $agmr18394
520 $aPlant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.
650 $aBreeding and Genetic Improvement
650 $aMelhoramento Vegetal
700 1 $aGUTIERREZ, D. L.
700 1 $aKREUZE, J. F.
700 1 $aISKRA-CARUANA, M. L.
700 1 $aCHABANNES, M.
700 1 $aBARBOSA, A. C. O.
700 1 $aSANTOS, T. A.
700 1 $aSILVA, A. G. S.
700 1 $aSANTOS, R. M. F.
700 1 $aAMORIM, E. P.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aJESUS, O. N. de
773 $tGenetics and Molecular Research$gv. 18, n.3, 2019.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Voltar
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
21/03/2001 |
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Data da última atualização: |
21/03/2001 |
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Autoria: |
FURUMOTO, O. CORDEIRO, C. M. T.; REIFSCHNEIDER, F. J. B. |
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Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
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Título: |
Ensaio nacional de cultivares de batata: teste avancado: relatorio 1/1983. |
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Ano de publicação: |
1983 |
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Fonte/Imprenta: |
Brasilia: EMBRAPA-CNPH, 1983. |
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Páginas: |
17p. |
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Idioma: |
Português |
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Palavras-Chave: |
Bahia; Brasil; Cultivar; Distrito Federal; Ensaio nacional; Espirito Santo; Goias; Mato Grosso; Mato Grosso do Sul; Minas Gerais; National trials; Paraiba; Parana; Rio de Janeiro; Rio Grande do Sul; Sao Paulo. |
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Thesagro: |
Batata; Cerrado; Solanum Tuberosum. |
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Thesaurus NAL: |
Brazil; potatoes; varieties. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00973nam a2200385 a 4500
001 1769892
005 2001-03-21
008 1983 bl uuuu u0uu1 u #d
100 1 $aFURUMOTO, O. CORDEIRO, C. M. T.
245 $aEnsaio nacional de cultivares de batata$bteste avancado: relatorio 1/1983.
260 $aBrasilia: EMBRAPA-CNPH$c1983
300 $a17p.
650 $aBrazil
650 $apotatoes
650 $avarieties
650 $aBatata
650 $aCerrado
650 $aSolanum Tuberosum
653 $aBahia
653 $aBrasil
653 $aCultivar
653 $aDistrito Federal
653 $aEnsaio nacional
653 $aEspirito Santo
653 $aGoias
653 $aMato Grosso
653 $aMato Grosso do Sul
653 $aMinas Gerais
653 $aNational trials
653 $aParaiba
653 $aParana
653 $aRio de Janeiro
653 $aRio Grande do Sul
653 $aSao Paulo
700 1 $aREIFSCHNEIDER, F. J. B.
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Embrapa Hortaliças (CNPH) |
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